Regulatory
Part:BBa_K641034:Design
Designed by: Nikit Patel Group: iGEM11_Berkeley (2011-09-26)
P(hdeAB) - Stress Promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 396
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 396
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 396
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 396
Illegal AgeI site found at 337 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Different stress promoters respond to different intra- and extracellular stress. Therefore it is key to test a variety of stress promoters and see what promoter gives the best stress response. We have put up many stress promoters for others to use and try.
Source
MG1655 Genomic DNA